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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
27/12/2017 |
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Data da última atualização: |
03/01/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
POLESE, V.; SOARES, C. de P.; SILVA, P. R. A. da; ARAUJO, J. L. S. de; BALDANI, J. I.; VIDAL, M. S. |
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Afiliação: |
VALÉRIA POLESE, UFRRJ; LEITON DE PAULA SOARES, UFRRJ; PAULA RENATA ALVES DA SILVA, UFRRJ; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; JOSE IVO BALDANI, CNPAB; MARCIA SOARES VIDAL, CNPAB. |
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Título: |
Selection and validation of reference genes for RT-qPCR indicates that juice of sugarcane varieties modulate the expression of C metabolism genes in the endophytic diazotrophic Herbaspirillum rubrisubalbicans strain HCC103. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Antonie van Leeuwenhoekv, 110, n. 12, p. 1555-1568, 2017. |
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ISSN: |
1572-9699 |
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DOI: |
https://doi.org/10.1007/s10482-017-0906-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Quantitative reverse transcription PCR (RT-qPCR) is an important tool for evaluating gene expression. However, this technique requires that specific internal normalizing genes be identified for different experimental conditions. To date, no internal normalizing genes are available for validation of data analyses for Herbaspirillum rubrisubalbicans strain HCC103, an endophyte that is part of the sugarcane consortium inoculant. This work seeks to identify and evaluate suitable reference genes for gene expression studies in HCC103 grown until middle log phase in sugarcane juice obtained from four sugarcane varieties or media with three different carbon sources. The mRNA levels of five candidate genes (rpoA, gyrA, dnaG, recA and gmK) and seven target genes involved in carbon metabolism (acnA, fbp, galE, suhB, wcaA, ORF_0127.0101 and _0127.0123) were quantified by RT-qPCR. Analysis of expression stability of these genes was carried out using geNorm and Normfinder software. The results indicated that the HCC103 dnaG and gyrA genes are the most stable and showed adequate relative expression level changes among the different sugarcane juices. The highest expression level was seen for ORF_0127.0101, which encodes a sugar transporter, in juice from sugarcane variety RB867515 and glucose as the carbon source. The suhB gene, encoding SuhB inositol monophosphatase, had a higher relative expression level on 0.5% glucose, 100% sugarcane juice from variety RB867515 and 0.5% aconitate. Together the results suggest that dnaG and gyrA genes are suitable as reference genes for RT-qPCR analysis of strain HCC103 and that juice from different sugarcane varieties modulates the expression of key genes involved in carbon metabolism. MenosQuantitative reverse transcription PCR (RT-qPCR) is an important tool for evaluating gene expression. However, this technique requires that specific internal normalizing genes be identified for different experimental conditions. To date, no internal normalizing genes are available for validation of data analyses for Herbaspirillum rubrisubalbicans strain HCC103, an endophyte that is part of the sugarcane consortium inoculant. This work seeks to identify and evaluate suitable reference genes for gene expression studies in HCC103 grown until middle log phase in sugarcane juice obtained from four sugarcane varieties or media with three different carbon sources. The mRNA levels of five candidate genes (rpoA, gyrA, dnaG, recA and gmK) and seven target genes involved in carbon metabolism (acnA, fbp, galE, suhB, wcaA, ORF_0127.0101 and _0127.0123) were quantified by RT-qPCR. Analysis of expression stability of these genes was carried out using geNorm and Normfinder software. The results indicated that the HCC103 dnaG and gyrA genes are the most stable and showed adequate relative expression level changes among the different sugarcane juices. The highest expression level was seen for ORF_0127.0101, which encodes a sugar transporter, in juice from sugarcane variety RB867515 and glucose as the carbon source. The suhB gene, encoding SuhB inositol monophosphatase, had a higher relative expression level on 0.5% glucose, 100% sugarcane juice from variety RB867515 and 0.5% aconitate. Toget... Mostrar Tudo |
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Palavras-Chave: |
Internal normalizing; Organic acids; Relative expression; Sugar transport. |
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Thesaurus Nal: |
nitrogen fixation. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02682naa a2200265 a 4500
001 2083708
005 2018-01-03
008 2017 bl uuuu u00u1 u #d
022 $a1572-9699
024 7 $ahttps://doi.org/10.1007/s10482-017-0906-7$2DOI
100 1 $aPOLESE, V.
245 $aSelection and validation of reference genes for RT-qPCR indicates that juice of sugarcane varieties modulate the expression of C metabolism genes in the endophytic diazotrophic Herbaspirillum rubrisubalbicans strain HCC103.$h[electronic resource]
260 $c2017
520 $aQuantitative reverse transcription PCR (RT-qPCR) is an important tool for evaluating gene expression. However, this technique requires that specific internal normalizing genes be identified for different experimental conditions. To date, no internal normalizing genes are available for validation of data analyses for Herbaspirillum rubrisubalbicans strain HCC103, an endophyte that is part of the sugarcane consortium inoculant. This work seeks to identify and evaluate suitable reference genes for gene expression studies in HCC103 grown until middle log phase in sugarcane juice obtained from four sugarcane varieties or media with three different carbon sources. The mRNA levels of five candidate genes (rpoA, gyrA, dnaG, recA and gmK) and seven target genes involved in carbon metabolism (acnA, fbp, galE, suhB, wcaA, ORF_0127.0101 and _0127.0123) were quantified by RT-qPCR. Analysis of expression stability of these genes was carried out using geNorm and Normfinder software. The results indicated that the HCC103 dnaG and gyrA genes are the most stable and showed adequate relative expression level changes among the different sugarcane juices. The highest expression level was seen for ORF_0127.0101, which encodes a sugar transporter, in juice from sugarcane variety RB867515 and glucose as the carbon source. The suhB gene, encoding SuhB inositol monophosphatase, had a higher relative expression level on 0.5% glucose, 100% sugarcane juice from variety RB867515 and 0.5% aconitate. Together the results suggest that dnaG and gyrA genes are suitable as reference genes for RT-qPCR analysis of strain HCC103 and that juice from different sugarcane varieties modulates the expression of key genes involved in carbon metabolism.
650 $anitrogen fixation
653 $aInternal normalizing
653 $aOrganic acids
653 $aRelative expression
653 $aSugar transport
700 1 $aSOARES, C. de P.
700 1 $aSILVA, P. R. A. da
700 1 $aARAUJO, J. L. S. de
700 1 $aBALDANI, J. I.
700 1 $aVIDAL, M. S.
773 $tAntonie van Leeuwenhoekv, 110$gn. 12, p. 1555-1568, 2017.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 9 | |
| 3. |  | POLESE, V.; SILVA, P. R. A.; TADRA-SFEIR, M. Z.; ARAUJO, J. L. S. de; SOUZA, E. M.; BALDANI, J. I.; VIDAL, M. S. Functional genomic of Herbaspirillum rubrisubalbicans HCC103 strain grown in the presence of sugarcane apoplastic liquid In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., 2015, Florianópolis. Resumos. Florianópolis: Sociedade Brasileira de Microbiologia, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 4. | | SILVA, P. R. A. da; VIDAL, M. S.; SOARES, C. de P.; POLESE, V.; ARAUJO, J. L. S. de; BALDANI, J. I. Selection and evaluation of reference genes for RT-qPCR expression studies on Burkholderia tropica strain Ppe8, a sugarcane-associated diazotrophic bacterium grown with different carbon sources or sugarcane juice Antonie van Leeuwenhoek, v. 109, n. 111, p. 1493-1502, 2016. Epub, 17 Aug., 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 5. | | POLESE, V.; SOARES, C. de P.; SILVA, P. R. A. da; ARAUJO, J. L. S. de; BALDANI, J. I.; VIDAL, M. S. Selection and validation of reference genes for RT-qPCR indicates that juice of sugarcane varieties modulate the expression of C metabolism genes in the endophytic diazotrophic Herbaspirillum rubrisubalbicans strain HCC103. Antonie van Leeuwenhoekv, 110, n. 12, p. 1555-1568, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 6. | | POLESE, V.; SILVA, P. R. A.; VALE, E.; HERINGER, A. S.; SOUZA FILHO, G. A. de; SILVEIRA, V.; BALDANI, J. I.; VIDAL, M. S. The effect of sugarcane apoplastic fluid on the proteome of HCC103 strain of Herbaspirillum rubrisubalbicans. In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 16., LATINAMERICAN WORKSHOP OF PGPR, 4., RELARE, 19., 2018, Foz do Iguaçu. Book of abstract....Foz do Iguaçu: Emprapa, 2018. p. 43 Session 4, Poster III.9| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 7. | | SILVA, P. R. A.; VIDAL, M. S.; POLESE, V.; TADRA-SFEIR, M. Z.; ARAUJO, J. L. S. de; SOUZA, E. M.; BALDANI, J. I. Differential gene expression of diazotrophic burkholderia tropica PPE5 strain cultivated in sugarcane apoplast fluid In: CONGRESSO BRASILEIRO DE GENÉTICA, 61., 2015, Águas de Lindóia. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 8. | | POLESE, V.; SILVA, P. R. A.; PESSOA, D. D. V.; TADRA-SFEIR, M. Z.; SOUZA, E. M.; ARAUJO, J. L. S. de; BALDANI, J. I.; VIDAL, M. S. Transcriptional profile of Herbaspirillum rubrisubalbicans in response of sugarcane apoplastic fluid. In: WORLD CONGRESS ON GENETICS APPLIED TO LIVESTOCK PRODUCTION, 11., 2018, Auckland, New Zealand. Proceedings... Massey University, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 9. | | SILVA, P. R. A. da; VIDAL, M. S.; SOARES, C. de P.; POLESE, V.; TADRA-SFEIR, M. Z.; SOUZA, E. M. de; ARAUJO, J. L. S. de; BALDANI, J. I. Sugarcane apoplast fluid modulates the global transcriptional profile of the diazotrophic bacteria Paraburkholderia tropica strain Ppe8. PLOS ONE, v. 13, n. 12, e0207863, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 9 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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